ARG62982

anti-Cytokeratin 8 antibody [C-51]

anti-Cytokeratin 8 antibody [C-51] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,ICC/IF and Human,Pig,Bovine,Sheep

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [C-51] recognizes Cytokeratin 8
Tested Reactivity Hu, Bov, Pig, Sheep
Species Does Not React With Ms, Rat, Chk, Dog, Rb, Xenopus laevis
Tested Application ICC/IF, IHC-P, IP, WB
Specificity The clone C-51 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-51
Isotype IgG1
Target Name Cytokeratin 8
Immunogen Cytoskeleton preparation from HeLa cells.
Conjugation Un-conjugated
Alternate Names Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P5 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281269 Bovine KRT8

GeneID: 3856 Human KRT8

Swiss-port # P05786 Bovine Keratin, type II cytoskeletal 8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

Gene Symbol KRT8
Gene Full Name keratin 8, type II
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 54 kDa
PTM Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Clone References

Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections.

ICC/IF / Human

Imbalzano KM et al.
PLoS One.,  (2013)

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Epithelial cell rests of Malassez contain unique stem cell populations capable of undergoing epithelial-mesenchymal transition.

IHC / Human

Xiong J et al.
Stem Cells Dev.,  (2012)

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Three clonal types of urothelium with different capacities for replication.

Thangappan R et al.
Cell Prolif.,  (2009)

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