ARG11075

anti-Cytokeratin 8 antibody [M20]

anti-Cytokeratin 8 antibody [M20] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Rabbit

Overview

Product Description Mouse Monoclonal antibody [M20] recognizes Cytokeratin 8
Tested Reactivity Hu, Rat, Rb
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone M20
Isotype IgG1
Target Name Cytokeratin 8
Antigen Species Human
Immunogen Keratin isolated from the Human breast carcinoma cell line MCF7.
Conjugation Un-conjugated
Alternate Names Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:100 - 1:200
IHC-P1:100 - 1:200
WB1:100 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 50 kDa

Properties

Form Liquid
Purification Purified
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25626 Rat KRT8

GeneID: 3856 Human KRT8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

Swiss-port # Q10758 Rat Keratin, type II cytoskeletal 8

Gene Symbol KRT8
Gene Full Name keratin 8, type II
Background This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Cellular Localization Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. [UniProt]
Calculated MW 54 kDa
PTM Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.

O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt]