ARG66196

anti-Cytokeratin 8 antibody [SQab1718]

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100 μl

355.00

50 μl

245.00

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Datasheet
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Overview

Product Description	Recombinant Rabbit Monoclonal antibody [SQab1718] recognizes Cytokeratin 8
Tested Reactivity	Hu
Tested Application	IHC-P, WB
Host	Rabbit
Clonality	Monoclonal
Clone	SQab1718
Target Name	Cytokeratin 8
Antigen Species	Human
Immunogen	Synthetic peptide around the C-terminus of Cytokeratin 8.
Conjugation	Un-conjugated
Alternate Names	Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8

Application Instructions

Application Suggestion

Tested Application	Dilution
IHC-P	1:200
WB	1:500

Application Note

IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative	0.01% Sodium azide
Stabilizer	40% Glycerol and 0.05% BSA
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 3856 Human KRT8 Swiss-port # P05787 Human Keratin, type II cytoskeletal 8
Gene Symbol	KRT8
Gene Full Name	keratin 8, type II
Background	This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Function	Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Highlight	Related news: Cancer Pathology Markers (SQ clones)
Calculated MW	54 kDa
PTM	Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.