ARG51782
anti-Cytokeratin 8 phospho (Ser74) antibody
anti-Cytokeratin 8 phospho (Ser74) antibody for Western blot,ICC/IF and Human
Cancer antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 8 phospho (Ser74) |
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Tested Reactivity | Hu |
Tested Application | ICC/IF, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Cytokeratin 8 |
Antigen Species | Human |
Immunogen | Peptide sequence around phosphorylation site of Ser74 (L-L-S(p)-P-L) derived from Human Cytokeratin 8 (CK8). |
Conjugation | Un-conjugated |
Alternate Names | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | KRT8 |
Gene Full Name | keratin 8, type II |
Background | Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. |
Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
Research Area | Cancer antibody; Signaling Transduction antibody |
Calculated MW | 54 kDa |
PTM | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |