ARG62586

anti-Cytokeratin (pan) antibody [B311.1]

anti-Cytokeratin (pan) antibody [B311.1] for ICC/IF,Immunoprecipitation,Immunohistochemistry,Western blot and Human,Mouse,Rat

Signaling Transduction antibody; Carcinoma Marker antibody

Overview

Product Description Mouse Monoclonal antibody [B311.1] recognizes Cytokeratin (pan)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, IP, WB
Host Mouse
Clonality Monoclonal
Clone B311.1
Isotype IgG1
Target Name Cytokeratin (pan)
Antigen Species Human
Immunogen Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
Conjugation Un-conjugated
Alternate Names 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHCAssay-dependent
IP1:2000
WB1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Skin, Colonic Carcinoma

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16678 Mouse KRT1

GeneID: 300250 Rat KRT1

GeneID: 3848 Human KRT1

Gene Symbol KRT1
Gene Full Name keratin 1, type II
Background The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt]
Research Area Signaling Transduction antibody; Carcinoma Marker antibody
Calculated MW 66 kDa
PTM Undergoes deimination of some arginine residues (citrullination).