ARG53924

anti-Cytokeratin (pan) antibody [C-11] (APC)

anti-Cytokeratin (pan) antibody [C-11] (APC) for Flow cytometry and Human,Mouse,Rat,Mammal

Signaling Transduction antibody; Carcinoma Marker antibody

Overview

Product Description Allophycocyanin-conjugated Mouse Monoclonal antibody [C-11] recognizes Cytokeratin (pan)
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application FACS
Specificity The clone C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-11
Isotype IgG1
Target Name Cytokeratin (pan)
Antigen Species Human
Immunogen Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Conjugation Allophycocyanin
Alternate Names 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16678 Mouse KRT1

GeneID: 300250 Rat KRT1

GeneID: 3848 Human KRT1

Gene Symbol KRT1
Gene Full Name keratin 1, type II
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt]
Research Area Signaling Transduction antibody; Carcinoma Marker antibody
Calculated MW 66 kDa
PTM Undergoes deimination of some arginine residues (citrullination).

Clone References

Intracellular distribution of p300 and its differential recruitment to aggresomes in breast cancer.

Fermento ME et al.
Exp Mol Pathol.,  (2010)

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Establishment and characterization of baboon embryonic stem cell lines: an Old World Primate model for regeneration and transplantation research.

ICC/IF / Baboon

Simerly CR et al.
Stem Cell Res.,  (2009)

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