ARG42331

anti-Cytokeratins antibody [C-11] (Biotin)

anti-Cytokeratins antibody [C-11] (Biotin) for Western blot,Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Mammal

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [C-11] recognizes Cytokeratins
Tested Reactivity Mamm
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Specificity The antibody C-11 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-11
Isotype IgG1
Target Name Cytokeratins
Antigen Species Human
Immunogen Keratin-enriched preparation from Human epidermoid carcinoma cell line A431.
Conjugation Biotin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol KRT4; KRT5; KRT6; KRT8; KRT10; KRT13; KRT18
Gene Full Name keratin 4, type I; keratin 5, type I; keratin 6, type I; keratin 8, type I; keratin 10, type I; keratin 13, type I; keratin 18, type I
Background The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Calculated MW 57 kDa