ARG63157
anti-DAPK2 antibody
anti-DAPK2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DAPK2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DAPK2 |
| Antigen Species | Human |
| Immunogen | C-KALHPRRRSSTS |
| Conjugation | Un-conjugated |
| Alternate Names | DRP1; DAP-kinase-related protein 1; DRP-1; DAP kinase 2; EC 2.7.11.1; Death-associated protein kinase 2 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 43 kDa |
| PTM | Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors. |
Images (3) Click the Picture to Zoom In
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ARG63157 anti-DAPK2 antibody WB image
Western Blot: mouse brain extracts (RIPA buffer, 35 µg total protein per lane) stained with ARG63157 anti-DAPK2 antibody at 2 µg/ml dilution.
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ARG63157 anti-DAPK2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63157 anti-DAPK2 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63157 anti-DAPK2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Breast. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63157 anti-DAPK2 antibody at 2.5 µg/ml dilution followed by AP-staining.
