ARG52254
anti-DARPP32 antibody
anti-DARPP32 antibody for Western blot,Immunohistochemistry,ICC/IF and Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes DARPP32 |
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Tested Reactivity | Rat |
Predict Reactivity | Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis |
Tested Application | ICC/IF, IHC, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | DARPP32 |
Antigen Species | Rat |
Immunogen | Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH |
Conjugation | Un-conjugated |
Alternate Names | Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B |
Application Instructions
Application Suggestion |
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Application Note | Specific for the ~32k DARPP-32 protein * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Affinity Purified |
Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q6J4I0 Rat Protein phosphatase 1 regulatory subunit 1B |
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Gene Symbol | PPP1R1B |
Gene Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 1B |
Background | DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002) |
Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
Calculated MW | 23 kDa |
PTM | Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity. |