ARG51680

anti-DARPP32 phospho (Thr75) antibody

anti-DARPP32 phospho (Thr75) antibody for ICC/IF and Human,Mouse,Rat

Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DARPP32 phospho (Thr75)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DARPP32
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of threonine 75 (A-Y-T(p)-P-P) derived from Human DARPP-32.
Conjugation Un-conjugated
Alternate Names Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19049 Mouse PPP1R1B

GeneID: 360616 Rat PPP1R1B

GeneID: 84152 Human PPP1R1B

Gene Symbol PPP1R1B
Gene Full Name protein phosphatase 1, regulatory (inhibitor) subunit 1B
Background Inhibitor of protein-phosphatase 1.
Function Inhibitor of protein-phosphatase 1. [UniProt]
Research Area Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 23 kDa
PTM Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.