ARG22860
anti-DAZL antibody [3/11A]
anti-DAZL antibody [3/11A] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Mouse Monoclonal antibody [3/11A] recognizes DAZL Mouse anti Human DAZL antibody, clone 3/11A recognizes human Deleted in azoospermia-like, also known as DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1 or SPGY-like-autosomal. DAZL is a 295 amino acid ~33 kDa member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is predominantly localized to the cytoplasm.Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3/11A |
| Isotype | IgG1 |
| Target Name | DAZL |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human DAZL. (CRVHHFRRSRAMLKSV) |
| Conjugation | Un-conjugated |
| Alternate Names | DAZL1; SPGYLA; Deleted in azoospermia-like; Deleted in azoospermia-like 1; SPGY-like-autosomal; DAZLA; DAZH; DAZ homolog; DAZ-like autosomal |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Tissue Culture Supernatant. |
| Buffer | Tissue Culture Supernatant and 0.09% Sodium azide, |
| Preservative | 0.09% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DAZL |
| Gene Full Name | deleted in azoospermia-like |
| Background | The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts (By similarity). [UniProt] |
| Calculated MW | 33 kDa |
