ARG63154

anti-DDB1 antibody

anti-DDB1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes DDB1
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name DDB1
Antigen Species Human
Immunogen C-DLIKVVEELTRIH
Conjugation Un-conjugated
Alternate Names XPCE; XPCe; DDB p127 subunit; DDBa; UV-damaged DNA-binding factor; HBV X-associated protein 1; DDBA; UV-damaged DNA-binding protein 1; XPE; XAP-1; Damage-specific DNA-binding protein 1; XPE-BF; DNA damage-binding protein a; UV-DDB1; UV-DDB 1; XAP1; Xeroderma pigmentosum group E-complementing protein; XPE-binding factor; DNA damage-binding protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB1 - 2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13194 Mouse DDB1

GeneID: 1642 Human DDB1

Swiss-port # Q16531 Human DNA damage-binding protein 1

Swiss-port # Q3U1J4 Mouse DNA damage-binding protein 1

Background The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]
Research Area Gene Regulation antibody
Calculated MW 127 kDa
PTM Phosphorylated by ABL1.
Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.