ARG23563

anti-DDDDK tag antibody [6F7] (HRP)

anti-DDDDK tag antibody [6F7] (HRP) for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody

Overview

Product Description HRP-conjugated Rat Monoclonal antibody [6F7] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies).
The antibody recognizes the hydrophilic epitope tagging peptide sequence DDDDK (Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins. This antibody works at neutral pH and has very low background staining in immunocytochemistry.
Tested Reactivity Other
Tested Application ELISA, IHC-P, WB
Host Rat
Clonality Monoclonal
Clone 6F7
Isotype IgG1
Target Name DDDDK tag
Antigen Species Others
Immunogen Synthetic peptide.
Conjugation HRP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
IHC-PAssay-dependent
WB1:100 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.01% Thiomersal and HRP Stabiliser.
Preservative 0.01% Thiomersal
Stabilizer HRP Stabiliser
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody