ARG62986

anti-DDDDK tag antibody [F-tag-01]

anti-DDDDK tag antibody [F-tag-01] for ICC/IF,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody

Overview

Product Description Mouse Monoclonal antibody [F-tag-01] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies).
Tested Reactivity Other
Tested Application ICC/IF, WB
Specificity The clone F-tag-01 recognizes DDDDK-tagged proteins in all species.
The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Host Mouse
Clonality Monoclonal
Clone F-tag-01
Isotype IgG1
Target Name DDDDK tag
Antigen Species Others
Immunogen KLH-conjugated synthetic peptide: DYKDDDDK
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related Antibody Duos and Panels:
ARG30003 Tag Internal Control Antibody Duo (DDDK tag, beta Actin)
Related products:
DDDDK tag antibodies; DDDDK tag Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Tag Internal Control antibody

Clone References

Investigation of membrane protein-protein interactions using correlative FRET-PLA.

Ivanusic D et al.
Biotechniques.,  (2014)

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Influenza polymerase encoding mRNAs utilize atypical mRNA nuclear export.

Larsen S et al.
Virol J.,  (2014)

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Phosphorylation of Nanog is essential to regulate Bmi1 and promote tumorigenesis.

Xie X et al.
Oncogene.,  (2014)

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Proton-assisted amino acid transporter PAT1 complexes with Rag GTPases and activates TORC1 on late endosomal and lysosomal membranes.

ICC/IF / 

Ögmundsdóttir MH et al.
PLoS One.,  (2012)

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Nuclear matrix binding site in the Rad51 recombinase.

WB, ICC/IF / 

Mladenov EV et al.
J Cell Physiol.,  (2009)

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