ARG66332

anti-DDDDK tag antibody [SQab1880]

anti-DDDDK tag antibody [SQab1880] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1880] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies).
The antibody recognizes the hydrophilic epitope tagging peptide sequence DDDDK (Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins.
Tested Reactivity Other
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1880
Isotype IgG
Target Name DDDDK tag
Antigen Species Others
Immunogen KLH-conjugated DYKDDDDK.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:1000 - 1:2000
ICC/IF1:1500 - 1:10000
IP1:50
WB1:10000 - 1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody