ARG58121
anti-DDX4 antibody
anti-DDX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DDX4 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DDX4 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-300 of Human DDX4 (NP_077726.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog; DEAD box protein 4; EC 3.6.4.13; MVH; Mouse Vasa Homologue |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse testis | ||||||||
| Observed Size | ~ 79 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q61496 Mouse Probable ATP-dependent RNA helicase DDX4 Swiss-port # Q64060 Rat Probable ATP-dependent RNA helicase DDX4 |
|---|---|
| Gene Symbol | DDX4 |
| Gene Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
| Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Function | May play a role in germ cell development. May play a role in sperm motility. [UniProt] |
| Cellular Localization | Cytoplasm, perinuclear region. Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. [UniProt] |
| Calculated MW | 79 kDa |
