ARG54342
anti-DFFB / DFF40 / CAD antibody
anti-DFFB / DFF40 / CAD antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DFFB / DFF40 / CAD |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody recognizes human, mouse, and rat DFFB / DFF40 / CAD (40 kDa). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DFFB / DFF40 / CAD |
| Antigen Species | Mouse |
| Immunogen | Peptide corresponding to aa. 147-164 near the center of mouse DFFB / DFF40 / CAD (accession no. NP_031885). The sequence differs from human DFFB / DFF40 / CAD by two amino acids. |
| Conjugation | Un-conjugated |
| Protein Full Name | DNA fragmentation factor subunit beta |
| Alternate Names | Caspase-activated DNase; DFF40; DFF-40; Caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; Caspase-activated nuclease; CPAN; DNA fragmentation factor subunit beta; DFF2; CAD |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | K562 and Jurkat |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Dffb |
| Gene Full Name | DNA fragmentation factor, beta subunit |
| Background | Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse Dnase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxy-ribonuclease). The human homologue of mouse CAD was more recently identified and termed CPAN, DFF40, and human CAD. DFF45/ICAD is the inhibitory protein of DFF40/CAD with which it forms complexes. Upon cleavage by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD is, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Function | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus [uniprot] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 39 kDa |
