ARG52258
anti-DGCR8 phospho (Ser377) antibody
anti-DGCR8 phospho (Ser377) antibody for Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DGCR8 phospho (Ser377) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DGCR8 |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | DOPA decarboxylase; AADC; DDC; Aromatic-L-amino-acid decarboxylase; EC 4.1.1.28 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~120k DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O88533 Mouse Aromatic-L-amino-acid decarboxylase Swiss-port # P20711 Human Aromatic-L-amino-acid decarboxylase |
|---|---|
| Gene Symbol | DGCR8 |
| Gene Full Name | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| Background | The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010). |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 54 kDa |
