ARG41176

anti-DHRS9 antibody

anti-DHRS9 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes DHRS9
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DHRS9
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 18-319 of Human DHRS9 (NP_001135743.1).
Conjugation Un-conjugated
Alternate Names Tracheobronchial epithelial cell-specific retinol dehydrogenase; 3-alpha-HSD; Short-chain dehydrogenase/reductase retSDR8; RDH-TBE; NADP-dependent retinol dehydrogenase/reductase; 3-alpha hydroxysteroid dehydrogenase; Dehydrogenase/reductase SDR family member 9; RDH15; RDHL; RDH-E2; RETSDR8; Short chain dehydrogenase/reductase family 9C member 4; Retinol dehydrogenase 15; RDHTBE; EC 1.1.-.-; SDR9C4; 3ALPHA-HSD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control THP-1
Observed Size 33 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10170 Human DHRS9

GeneID: 170635 Rat DHRS9

GeneID: 241452 Mouse DHRS9

Gene Symbol DHRS9
Gene Full Name dehydrogenase/reductase (SDR family) member 9
Background This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function 3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH. [UniProt]
Cellular Localization Microsome membrane. Endoplasmic reticulum membrane. Note=Associated with microsomal membranes. [UniProt]
Calculated MW 35 kDa