ARG42699

anti-DHX15 / Prp43 antibody

anti-DHX15 / Prp43 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes DHX15 / Prp43
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DHX15 / Prp43
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence of Human DHX15 / Prp43. (DIKPEWLVKIAPQYYDMSNFPQCEAKRQLDRIIAKLQSKEYSQY)
Conjugation Un-conjugated
Alternate Names Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; DDX15; PrPp43p; ATP-dependent RNA helicase #46; PRP43; EC 3.6.4.13; DEAH box protein 15; HRH2; DBP1; PRPF43

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 91 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13204 Mouse DHX15

GeneID: 1665 Human DHX15

Swiss-port # O35286 Mouse Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Swiss-port # O43143 Human Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Gene Symbol DHX15
Gene Full Name DEAH (Asp-Glu-Ala-His) box helicase 15
Background The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing. [provided by RefSeq, Jul 2008]
Function Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. [UniProt]
Cellular Localization Nucleus. Nucleus, nucleolus. [UniProt]
Calculated MW 91 kDa