ARG58553
anti-DIAPH2 phospho (Thr1061) antibody
anti-DIAPH2 phospho (Thr1061) antibody for Western blot and Human,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DIAPH2 phospho (Thr1061) |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | WB |
| Specificity | This antibody detects a ~135 kDa protein corresponding to DIAPH2 in immunoblots of human A7r5 and mouse C2C12 cells treated with calyculin A. This antibody detects transfected DIAPH2 after treatment with calyculin A, but this reactivity is removed after phosphatase treatment. In addition, the antibody does not detect DIAPH2 (T1061A) mutant. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DIAPH2 |
| Antigen Species | Mouse |
| Immunogen | KLH-conjugated phospho specific peptide around Thr1061 of Mouse DIAPH2. This peptide sequence is identical in Rat and has 2 aa. differences to Human DIAPH2. The sequence has low homology to other formins. |
| Conjugation | Un-conjugated |
| Alternate Names | Diaphanous-related formin-2; DRF2; Protein diaphanous homolog 2; POF2; DIA; POF; DIA2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 135 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 50% Glycerol and 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DIAPH2 |
| Gene Full Name | diaphanous-related formin 2 |
| Background | The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Function | Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. [UniProt] |
| Calculated MW | 126 kDa |
