ARG40038
anti-DNA Ligase 3 antibody
anti-DNA Ligase 3 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DNA Ligase 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DNA Ligase 3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 730-1009 of Human DNA Ligase 3 (NP_039269.2). |
| Conjugation | Un-conjugated |
| Alternate Names | DNA ligase III; EC 6.5.1.1; Polydeoxyribonucleotide synthase [ATP] 3; LIG2; DNA ligase 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 112 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LIG3 |
| Gene Full Name | ligase III, DNA, ATP-dependent |
| Background | This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair. [UniProt] |
| Cellular Localization | Isoform 1: Mitochondrion. Note=Contains an N-terminal mitochondrial transit peptide. Isoform 2: Mitochondrion. Note=Contains an N-terminal mitochondrial transit peptide. Isoform 3: Nucleus. Note=Lacks the N-terminal mitochondrial transit peptide. Isoform 4: Nucleus. Note=Lacks the N-terminal mitochondrial transit peptide. [UniProt] |
| Calculated MW | 113 kDa |
