ARG62471

anti-DNA PKcs antibody [18-2]

anti-DNA PKcs antibody [18-2] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Inhibition assay and Mouse,Rat,Human

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [18-2] recognizes DNA PKcs
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, Inhib, WB
Host Mouse
Clonality Monoclonal
Clone 18-2
Isotype IgG1
Target Name DNA PKcs
Antigen Species Human
Immunogen Human DNA-dependent Protein Kinase purified from HeLa cells
Epitope aa 1-2713
Conjugation Un-conjugated
Alternate Names p350; DNAPK; HYRC1; DNA-PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; IMD26; HYRC; EC 2.7.11.1; p460; DNA-PK catalytic subunit; XRCC7

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P1:400
IP1:400
InhibAssay-dependent
WB1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control LS174T cells. Tonsil.

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19090 Mouse PRKDC

GeneID: 5591 Human PRKDC

Swiss-port # P78527 Human DNA-dependent protein kinase catalytic subunit

Swiss-port # P97313 Mouse DNA-dependent protein kinase catalytic subunit

Gene Symbol PRKDC
Gene Full Name protein kinase, DNA-activated, catalytic polypeptide
Background This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 469 kDa
PTM Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
S-nitrosylated by GAPDH.
Polyubiquitinated by RNF144A, leading to proteasomal degradation.

Clone References

Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.

Keka IS et al.
Nucleic Acids Res.,  (2015)

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Quantitation of DNA double-strand break resection intermediates in human cells.

WB / Human

Zhou Y et al.
Nucleic Acids Res.,  (2014)

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The influence of AKT isoforms on radiation sensitivity and DNA repair in colon cancer cell lines.

ICC/IF / Human

Sahlberg SH et al.
Tumour Biol.,  (2014)

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Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.

WB / Human

Kang KB et al.
Int J Radiat Oncol Biol Phys.,  (2012)

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EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment.

WB, ICC/IF / Mouse

Liccardi G et al.
Cancer Res.,  (2011)

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Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.

IP / Human

Schild-Poulter C et al.
Rheumatology (Oxford).,  (2008)

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