ARG62471
anti-DNA PKcs antibody [18-2]
anti-DNA PKcs antibody [18-2] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Inhibition assay and Mouse,Rat,Human
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [18-2] recognizes DNA PKcs |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, Inhib, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 18-2 |
| Isotype | IgG1 |
| Target Name | DNA PKcs |
| Antigen Species | Human |
| Immunogen | Human DNA-dependent Protein Kinase purified from HeLa cells |
| Epitope | aa 1-2713 |
| Conjugation | Un-conjugated |
| Alternate Names | p350; DNAPK; HYRC1; DNA-PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; IMD26; HYRC; EC 2.7.11.1; p460; DNA-PK catalytic subunit; XRCC7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||||
| Positive Control | LS174T cells. Tonsil. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P78527 Human DNA-dependent protein kinase catalytic subunit Swiss-port # P97313 Mouse DNA-dependent protein kinase catalytic subunit |
|---|---|
| Gene Symbol | PRKDC |
| Gene Full Name | protein kinase, DNA-activated, catalytic polypeptide |
| Background | This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010] |
| Function | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 469 kDa |
| PTM | Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. S-nitrosylated by GAPDH. Polyubiquitinated by RNF144A, leading to proteasomal degradation. |
Clone References
Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.
Quantitation of DNA double-strand break resection intermediates in human cells.
WB / Human
The influence of AKT isoforms on radiation sensitivity and DNA repair in colon cancer cell lines.
ICC/IF / Human
Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.
WB / Human
EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment.
WB, ICC/IF / Mouse
Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.
IP / Human
