ARG43061

anti-DNAJA2 antibody

anti-DNAJA2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes DNAJA2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DNAJA2
Antigen Species Human
Immunogen Recombinant protein corresponding to M1-N140 of Human DNAJA2.
Conjugation Un-conjugated
Alternate Names Dnj3; DJ3; Dj3; Cell cycle progression restoration gene 3 protein; DnaJ homolog subfamily A member 2; PRO3015; RDJ2; DJA2; HIRA-interacting protein 4; DNJ3; HIRIP4; DNAJ; CPR3; Renal carcinoma antigen NY-REN-14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10294 Human DNAJA2

GeneID: 56445 Mouse DNAJA2

Swiss-port # O60884 Human DnaJ homolog subfamily A member 2

Swiss-port # Q9QYJ0 Mouse DnaJ homolog subfamily A member 2

Gene Symbol DNAJA2
Gene Full Name DnaJ (Hsp40) homolog, subfamily A, member 2
Background The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008]
Function Co-chaperone of Hsc70. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877). [UniProt]
Cellular Localization Membrane; Lipid-anchor. [UniProt]
Calculated MW 46 kDa