ARG66719

anti-DNAJC5 / CSP antibody

anti-DNAJC5 / CSP antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes DNAJC5 / CSP
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DNAJC5 / CSP
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 131-180 of Human DNAJC5 / CSP.
Conjugation Un-conjugated
Alternate Names mir-941-5; mir-941-4; CLN4B; Ceroid-lipofuscinosis neuronal protein 4; mir-941-3; mir-941-2; DNAJC5A; Cysteine string protein; DnaJ homolog subfamily C member 5; CLN4; NCL; CSP

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 22 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80331 Human DNAJC5

Swiss-port # Q9H3Z4 Human DnaJ homolog subfamily C member 5

Gene Symbol DNAJC5
Gene Full Name DnaJ (Hsp40) homolog, subfamily C, member 5
Background This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of this gene is located on the short arm of chromosome 8. [provided by RefSeq, Nov 2010]
Function Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity). [UniProt]
Cellular Localization Membrane; Lipid-anchor. Melanosome. Cell membrane. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt]
Calculated MW 22 kDa
PTM Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity).

Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58 (PubMed:27452402). This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions (PubMed:27452402). [UniProt]