ARG66719
anti-DNAJC5 / CSP antibody
anti-DNAJC5 / CSP antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DNAJC5 / CSP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DNAJC5 / CSP |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 131-180 of Human DNAJC5 / CSP. |
| Conjugation | Un-conjugated |
| Alternate Names | mir-941-5; mir-941-4; CLN4B; Ceroid-lipofuscinosis neuronal protein 4; mir-941-3; mir-941-2; DNAJC5A; Cysteine string protein; DnaJ homolog subfamily C member 5; CLN4; NCL; CSP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | ~ 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DNAJC5 |
| Gene Full Name | DnaJ (Hsp40) homolog, subfamily C, member 5 |
| Background | This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. The encoded protein plays a role in membrane trafficking and protein folding, and has been shown to have anti-neurodegenerative properties. The encoded protein is known to play a role in cystic fibrosis and Huntington's disease. A pseudogene of this gene is located on the short arm of chromosome 8. [provided by RefSeq, Nov 2010] |
| Function | Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity). [UniProt] |
| Cellular Localization | Membrane; Lipid-anchor. Melanosome. Cell membrane. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity). Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58 (PubMed:27452402). This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions (PubMed:27452402). [UniProt] |
