ARG63676

anti-DOK5 antibody

anti-DOK5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes DOK5
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name DOK5
Antigen Species Human
Immunogen CLHRTETFPAYRSEH
Conjugation Un-conjugated
Alternate Names IRS6; IRS-6; Docking protein 5; C20orf180; Insulin receptor substrate 6; Downstream of tyrosine kinase 5

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
WB1 - 3 µg/ml
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55816 Human DOK5

Swiss-port # Q9P104 Human Docking protein 5

Background The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 35 kDa
PTM Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.