ARG62472

anti-DP1 antibody

anti-DP1 antibody for Western blot,Immunoprecipitation and Human,Mouse,Rat

Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DP1
Tested Reactivity Hu, Ms, Rat
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DP1
Antigen Species Human
Immunogen Synthetic peptide from the N terminus of human DP-1 protein.
Epitope N-terminal
Conjugation Un-conjugated
Alternate Names DRTF1-polypeptide 1; DP1; DRTF1; E2F dimerization partner 1; Transcription factor Dp-1; Dp-1

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:2000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431 or Raji cells

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21781 Mouse TFDP1

GeneID: 7027 Human TFDP1

Swiss-port # Q08639 Mouse Transcription factor Dp-1

Swiss-port # Q14186 Human Transcription factor Dp-1

Gene Symbol TFDP1
Gene Full Name transcription factor Dp-1
Background This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009]
Function Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812). [UniProt]
Research Area Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 45 kDa
PTM Phosphorylation by E2F1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.
Ubiquitinated by the BCR(KBTBD5) complex, leading to its subsequent degradation.