ARG58514

anti-DRD3 / Dopamine Receptor D3 antibody

anti-DRD3 / Dopamine Receptor D3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes DRD3 / Dopamine Receptor D3
Tested Reactivity Hu, Rat
Predict Reactivity Chk
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DRD3 / Dopamine Receptor D3
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence at the C-terminus of Human Dopamine Receptor D3(352-367aa NTHCQTCHVSPELYSA), different from the related Rat and Mouse sequences by two amino acids.
Conjugation Un-conjugated
Alternate Names D3DR; ETM1; FET1; D(3) dopamine receptor; Dopamine D3 receptor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1814 Human DRD3

GeneID: 29238 Rat DRD3

Swiss-port # P19020 Rat D(3) dopamine receptor

Swiss-port # P35462 Human D(3) dopamine receptor

Gene Symbol DRD3
Gene Full Name dopamine receptor D3
Background This gene encodes the D3 subtype of the five (D1-D5) dopamine receptors. The activity of the D3 subtype receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. Genetic variation in this gene may be associated with susceptibility to hereditary essential tremor 1. Alternative splicing of this gene results in transcript variants encoding different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008]
Function Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells. [UniProt]
Calculated MW 44 kDa
PTM Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma). [UniProt]