ARG63190

anti-DUSP10 / MKP5 antibody

anti-DUSP10 / MKP5 antibody for Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes DUSP10 / MKP5
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application WB
Specificity This antibody is expected to recognise both reported Human isofoms. The variants NP_653329.1 and NP_653330.1 represent identical protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name DUSP10 / MKP5
Antigen Species Human
Immunogen CRILTPKLMGVETVV
Conjugation Un-conjugated
Alternate Names MKP-5; Dual specificity protein phosphatase 10; MKP5; MAP kinase phosphatase 5; EC 3.1.3.16; Mitogen-activated protein kinase phosphatase 5; EC 3.1.3.48

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5 - 2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11221 Human DUSP10

Swiss-port # Q9Y6W6 Human Dual specificity protein phosphatase 10

Background Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 53 kDa