ARG63527

anti-DUSP16 antibody

anti-DUSP16 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes DUSP16
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application IHC-P
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name DUSP16
Antigen Species Human
Immunogen AHEMIGTQIVTER-C
Conjugation Un-conjugated
Alternate Names MKP-7; Dual specificity protein phosphatase 16; MAP kinase phosphatase 7; EC 3.1.3.16; Mitogen-activated protein kinase phosphatase 7; EC 3.1.3.48; MKP7

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80824 Human DUSP16

Swiss-port # Q9BY84 Human Dual specificity protein phosphatase 16

Background This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]
Research Area Signaling Transduction antibody
Calculated MW 73 kDa
PTM Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity.
(Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.