ARG23468
anti-Dendritic Cells antibody [YTH862.2]
anti-Dendritic Cells antibody [YTH862.2] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human
Overview
| Product Description | Rat Monoclonal antibody [YTH862.2] recognizes Dendritic Cells Rat anti Human HLA ABC antibody, clone YTH862.2 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.Rat anti Human HLA ABC antibody, clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997). |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IP |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YTH862.2 |
| Isotype | IgG2b |
| Target Name | Dendritic Cells |
| Antigen Species | Human |
| Immunogen | PHA activated human peripheral blood lymphocytes. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | Functional study: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
