ARG43208

anti-Desmin antibody [DE-U-10]

anti-Desmin antibody [DE-U-10] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [DE-U-10] recognizes Desmin
Tested Reactivity Ms, Rat
Predict Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone DE-U-10
Isotype IgG1
Target Name Desmin
Antigen Species Pig
Immunogen Desmin from pig stomach.
Conjugation Un-conjugated
Alternate Names LGMD2R; CSM1; Desmin; CSM2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P2 - 4 µg/ml
WB2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 54 kDa

Properties

Form Liquid
Purification Unpurified
Buffer Mouse ascites fluid, 1.2% Sodium acetate, 0.01 mg Sodium azide and 2 mg BSA.
Preservative 0.01 mg Sodium azide
Stabilizer 2 mg BSA
Concentration 0.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13346 Mouse DES

GeneID: 64362 Rat DES

Swiss-port # P31001 Mouse Desmin

Swiss-port # P48675 Rat Desmin

Gene Symbol DES
Gene Full Name desmin
Background This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
Function Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity). [UniProt]
Cellular Localization Cytoplasm, myofibril, sarcomere, Z line. Cytoplasm. Cell membrane, sarcolemma. Nucleus. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190). Localizes in the nucleus exclusively in differentiating cardiac progenitor cells and premature cardiomyocytes (By similarity). [UniProt]
Calculated MW 54 kDa
PTM ADP-ribosylation prevents ability to form intermediate filaments. [UniProt]