ARG52943

anti-Desmin antibody [SP138]

anti-Desmin antibody [SP138] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
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Overview

Product Description

Rabbit Monoclonal antibody [SP138] recognizes Desmin

Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog, Pig
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP138
Isotype IgG
Target Name Desmin
Antigen Species Human
Immunogen Synthetic peptide derived from the C-terminus of human desmin protein.
Conjugation Un-conjugated
Alternate Names LGMD2R; CSM1; Desmin; CSM2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC-P1:100
WB1:100
Application Note IHC-P: Antigen Retrieval: None.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Skeletal Muscle, Human Skeletal Muscle

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13346 Mouse DES

GeneID: 1674 Human DES

Swiss-port # P17661 Human Desmin

Swiss-port # P31001 Mouse Desmin

Gene Full Name desmin
Background Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
Function Desmin: Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin. [UniProt]
Cellular Localization Cytoplasm
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
Calculated MW 54 kDa
PTM ADP-ribosylation prevents ability to form intermediate filaments.

Customer's Feedback

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Review for anti-Desmin antibody [SP138]

Application:WB

Sample:Rat heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC