ARG58560

anti-Desmocollin 2 + 3 antibody [7G6]

anti-Desmocollin 2 + 3 antibody [7G6] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry,ICC/IF and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [7G6] recognizes Desmocollin 2 + 3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 7G6
Isotype IgG1, kappa
Target Name Desmocollin 2 + 3
Antigen Species Human
Immunogen Recombinant protein corresponding to the extracellular domain of Human Desmocollin 2.
Conjugation Un-conjugated
Alternate Names Desmosomal glycoprotein II; ARVD11; Desmosomal glycoprotein III; DSC3; Desmocollin-3; Desmocollin-2; DG2; CDHF2; DGII/III; Cadherin family member 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 5 µg/ml
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13506 Mouse DSC2

GeneID: 1824 Human DSC2

Swiss-port # P55292 Mouse Desmocollin-2

Swiss-port # Q02487 Human Desmocollin-2

Gene Symbol DSC2
Gene Full Name desmocollin 2
Background This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Function Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. [UniProt]
Cellular Localization Cell surface. [UniProt]
Calculated MW 100 kDa