ARG23200
anti-Desmoplakin I+II antibody [DP-2.15]
anti-Desmoplakin I+II antibody [DP-2.15] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Bovine,Chicken
Overview
| Product Description | Mouse Monoclonal antibody [DP-2.15] recognizes Desmoplakin DPI and DPII Mouse anti Desmoplakin 1/2 antibody, clone DP-2. 15 recognizes both desmoplakin 1 and 2 from stratified epithelia, simple epithelia including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges. Mouse anti Desmoplakin 1/2 antibody, clone DP-2. 15 can be used for the detection of primary and metastatic carcinomas. Mouse anti Desmoplakin 1/2 antibody, clone DP-2. 15 recognizes the N terminus of the rod domain of desmoplakin ( Bornslaeger et al. 1996). |
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| Tested Reactivity | Hu, Ms, Rat, Bov, Chk |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DP-2.15 |
| Isotype | IgG1 |
| Target Name | Desmoplakin I+II |
| Antigen Species | Human |
| Immunogen | Native Bovine desmoplakin 1 and 2 purified from Bovine snout desmosomes. |
| Conjugation | Un-conjugated |
| Alternate Names | 250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin; DP; DPII; DCWHKTA; DPI |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
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| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | DSP |
| Gene Full Name | desmoplakin |
| Background | Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008] |
| Function | Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. [UniProt] |
| Calculated MW | 332 kDa |
| PTM | Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments. [UniProt] |
