ARG22285
anti-Dnmt3L antibody [S117-9]
anti-Dnmt3L antibody [S117-9] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [S117-9] recognizes Dnmt3L |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC, IP, WB |
| Specificity | Detects ~45kDa. No cross-reactivity against DNMT3a or DNMT3B. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S117-9 |
| Isotype | IgG1 |
| Target Name | Dnmt3L |
| Antigen Species | Human |
| Immunogen | Fusion protein around aa. 1-387 of Human Dnmt3L |
| Conjugation | Un-conjugated |
| Alternate Names | DNA (cytosine-5)-methyltransferase 3-like |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9CWR8 Mouse DNA (cytosine-5)-methyltransferase 3-like Swiss-port # Q9UJW3 Human DNA (cytosine-5)-methyltransferase 3-like |
|---|---|
| Gene Symbol | DNMT3L |
| Gene Full Name | DNA (cytosine-5-)-methyltransferase 3-like |
| Background | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
| Function | Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. [UniProt] |
| Cellular Localization | Nucleus |
| Calculated MW | 44 kDa |
