ARG56553

anti-Dnmt3a antibody

anti-Dnmt3a antibody for Western blot,ICC/IF and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Dnmt3a
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Dnmt3a
Antigen Species Human
Immunogen Recombinant protein of Human Dnmt3a.
Conjugation Un-conjugated
Alternate Names TBRS; DNMT3A2; M.HsaIIIA; DNA (cytosine-5)-methyltransferase 3A; Dnmt3a; EC 2.1.1.37; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SW620

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1788 Human DNMT3A

Swiss-port # Q9Y6K1 Human DNA (cytosine-5)-methyltransferase 3A

Gene Symbol DNMT3A
Gene Full Name DNA (cytosine-5-)-methyltransferase 3 alpha
Background CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]
Calculated MW 102 kDa
PTM Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.