ARG52265

anti-Dopamine Transporter phospho (Thr53) antibody

anti-Dopamine Transporter phospho (Thr53) antibody for Western blot,Immunohistochemistry and Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Dopamine Transporter phospho (Thr53)
Tested Reactivity Rat
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Dopamine Transporter
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH
Conjugation Un-conjugated
Alternate Names PKDYS; Sodium-dependent dopamine transporter; Solute carrier family 6 member 3; DAT1; DAT; DA transporter

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WB1:1,000
Application Note Specific for the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling of the DAT band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24898 Rat SLC6A3

Swiss-port # P23977 Rat Sodium-dependent dopamine transporter

Gene Symbol SLC6A3
Gene Full Name solute carrier family 6 (neurotransmitter transporter), member 3
Background The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr53 residue plays a major role in transport activity (Foster et al., 2012).
Highlight Related Antibody Duos and Panels:
ARG30102 Phospho Dopamine Transporter Antibody Duo (Total, pT53)
Related products:
Dopamine Transporter antibodies; Dopamine Transporter Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Neuroscience antibody
Calculated MW 68 kDa