ARG42043

anti-Dopamine beta Hydroxylase antibody

anti-Dopamine beta Hydroxylase antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Dopamine beta Hydroxylase
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Dopamine beta Hydroxylase
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide between aa. 27-56 of Human Dopamine beta Hydroxylase.
Conjugation Un-conjugated
Alternate Names EC 1.14.17.1; DBM; Dopamine beta-monooxygenase; Dopamine beta-hydroxylase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:25
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat adrenal gland
Observed Size ~ 75 kDa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13166 Mouse DBH

GeneID: 1621 Human DBH

GeneID: 25699 Rat DBH

Gene Symbol DBH
Gene Full Name dopamine beta-hydroxylase (dopamine beta-monooxygenase)
Background The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]
Function Conversion of dopamine to noradrenaline. [UniProt]
Cellular Localization Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein. [UniProt]
Calculated MW 69 kDa
PTM N-glycosylated.

Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form. [UniProt]