ARG52269
anti-Dynamin phospho (Ser774) antibody
anti-Dynamin phospho (Ser774) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Sheep Polyclonal antibody recognizes Dynamin phospho (Ser774) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Chk, Dog, NHuPrm |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dynamin |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | DNM; EC 3.6.5.5; Dynamin-1; EIEE31 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substRates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DNM1 |
| Gene Full Name | dynamin 1 |
| Background | Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser774 by GSK3 has recently been shown to control activitydependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010) |
| Highlight | Related Antibody Duos and Panels: ARG30101 Phospho Dynamin Antibody Duo (Total, pS774) Related products: Dynamin antibodies; Dynamin Duos / Panels; Anti-Sheep IgG secondary antibodies; |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 97 kDa |
