ARG22409
anti-Dynorphin A antibody
anti-Dynorphin A antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Radioimmunoassay and Human,Rat,Pig,Rhesus Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Dynorphin A This antibody recognizes the opioid peptide dynorphin A (Fischli et al. 1982), a cleavage product of big dynorphin, itself derived from the precursor pro-encephalin-B, also known as preprodynorphin. Rabbit anti human dynorphin A antibody binds to both dynorphin A 1-17 and 1-8 but not with other big dynorphin cleavage products such as dynorphin B, also known as rimorphin. Dynorphin A immunoreactive cells are found in the paraventricular and supraoptic nucleus and in hippocampal mossy fibers of rat brain following kainic acid administration and colchicine induced neurotoxicity (McGinty et al. 1983). Dynorphins play a role in multiple physiologic processes including pain perception (Wang et al. 2001)and responses to stress (Rácz et al. 2013). They mimic and compete with a number of opiate drugs(Chavkin 2013). Dysregulation of dynorphin A expression has been linked to progression of neurodegenerative conditions such as Altzheimers disease (Yakovleva et al. 2007) and appears to play a significant role in the pathobiology of epilepsy (Loacker et al. 2007). Rabbit anti human dynorphin antibody has been used successfully for the immunohistochemical demonstration of mossy fibre sprouting in hippocampal sclerosis (Thom et al. 2009) and in a virally induced disease model in the rat (Solbrig et al. 2006). |
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| Tested Reactivity | Hu, Rat, Pig, R. Mk |
| Tested Application | IHC-Fr, IHC-P, RIA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dynorphin A |
| Antigen Species | Human |
| Immunogen | Dynorphin A 1-17: Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln (porcine) |
| Conjugation | Un-conjugated |
| Alternate Names | Big Dyn; 1-8; Dynorphin B-29; Dynorphin B; ADCA; SCA23; Preprodynorphin; PENKB; Dyn-A17; 1-17; Proenkephalin-B; Dynorphin A; 1-13; Beta-neoendorphin-dynorphin; Dyn-B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Overnight incubation is recommended for paraffin sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Serum |
| Buffer | PBS, 0.09% Sodium azide and 0.1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | PDYN |
| Gene Full Name | prodynorphin |
| Background | The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (By similarity). Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin (By similarity). Leumorphin has a typical opiod activity and may have anti-apoptotic effect. [UniProt] |
| Calculated MW | 28 kDa |
| PTM | The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. |
