ARG55914

Name: anti-E Cadherin antibody
Brand: arigo Biolaboratories
SKU: ARG55914
Price: 355 USD
Availability: InStock
Rating: 4.3 (3 reviews)

anti-E Cadherin antibody

Publication1

Ordering Information

Package

Price (USD)

100 μl

355.00

50 μl

245.00

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Overview

Product Description	Mouse Monoclonal antibody recognizes E Cadherin
Tested Reactivity	Hu, Ms
Tested Application	WB
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Target Name	E Cadherin
Conjugation	Un-conjugated
Alternate Names	Uvomorulin; Arc-1; Cadherin-1; E-cadherin; CDHE; CD antigen CD324; ECAD; CAM 120/80; LCAM; Epithelial cadherin; UVO; CD324

Application Instructions

Application Suggestion

Tested Application	Dilution
WB	1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Observed Size

135 kDa (precursor)

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.4), 0.03% Proclin-300 and 50% Glycerol
Preservative	0.03% Proclin-300
Stabilizer	50% Glycerol
Concentration	2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12550 Mouse CDH1 GeneID: 999 Human CDH1 Swiss-port # P09803 Mouse Cadherin-1 Swiss-port # P12830 Human Cadherin-1
Gene Symbol	CDH1
Gene Full Name	cadherin 1, type 1
Background	E Cadherin is a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Function	Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. [UniProt]
Cellular Localization	Cell junction; Cell membrane; Single-pass type I membrane protein; Endosome; Golgi apparatus > trans-Golgi network.
Research Area	EMT Study antibody; Epithelial Marker antibody
Calculated MW	97 kDa
PTM	During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.