ARG62474

anti-E2F1 antibody [KH20]

anti-E2F1 antibody [KH20] for Western blot,Immunoprecipitation,Immunohistochemistry,Gel supershift assays and Human,Rat,Xenopus laevis,Mouse

Cancer antibody; Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [KH20] recognizes E2F1
Tested Reactivity Hu, Ms, Rat, Xenopus laevis
Tested Application GSA, IHC, IP, WB
Specificity The epitope recognized by the antibody resides in the N-terminal region of human E2F1 (amino acids 1-89).
Host Mouse
Clonality Monoclonal
Clone KH20
Isotype IgG2a
Target Name E2F1
Antigen Species Human
Immunogen Recombinant human E2F1
Conjugation Un-conjugated
Alternate Names RBAP1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; RBBP3; pRB-binding protein E2F-1; RBBP-3; E2F-1; Transcription factor E2F1; RBP3; PBR3; RBAP-1

Application Instructions

Application Note WB: 1-2 ug/ml
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.01 M PBS (pH 7.4), 1% BSA and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Stabilizer 1% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13555 Mouse E2F1

GeneID: 1869 Human E2F1

GeneID: 399489 Rat E2F1

Gene Symbol E2F1
Gene Full Name E2F transcription factor 1
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). [UniProt]
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 47 kDa
PTM Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.