ARG43665
anti-E2F6 antibody
anti-E2F6 antibody for ChIP,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes E2F6 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ChIP, ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | E2F6 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human E2F6 |
| Conjugation | Un-conjugated |
| Protein Full Name | Transcription factor E2F6 |
| Alternate Names | E2F-6; Transcription factor E2F6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | ~32 - 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | E2F6 |
| Gene Full Name | E2F transcription factor 6 |
| Background | This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Function | Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 32 kDa |
| PTM | Isopeptide bond; Ubl conjugation [UniProt] |
