ARG64430

anti-EAT2 phospho (Tyr127) antibody

anti-EAT2 phospho (Tyr127) antibody for Western blot and Human

Immune System antibody

Overview

Product Description Goat Polyclonal antibody recognizes EAT2 phospho (Tyr127)
Tested Reactivity Hu
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name EAT2
Antigen Species Human
Immunogen C-NSNSDpYVDVLP
Conjugation Un-conjugated
Alternate Names EAT-2; EWS/FLI1-activated transcript 2; EAT2; SH2 domain-containing protein 1B

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Subsequently passed through a column with the non-phosphorylated version of the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117157 Human SH2D1B

Swiss-port # O14796 Human SH2 domain-containing protein 1B

Background By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008]
Research Area Immune System antibody
Calculated MW 15 kDa