ARG55949

anti-ECM1 / Secretory Component antibody [SC05]

anti-ECM1 / Secretory Component antibody [SC05] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Rat

Overview

Product Description Mouse Monoclonal antibody [SC05] recognizes ECM1 / Secretory Component
Tested Reactivity Hu, Rat
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone SC05
Isotype IgG1, kappa
Target Name ECM1 / Secretory Component
Antigen Species Human
Immunogen Secretory component protein isolated from Human colostrum.
Conjugation Un-conjugated
Alternate Names URBWD; Extracellular matrix protein 1; Secretory component p85

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116662 Rat ECM1

GeneID: 1893 Human ECM1

Swiss-port # Q16610 Human Extracellular matrix protein 1

Swiss-port # Q62894 Rat Extracellular matrix protein 1

Gene Symbol ECM1
Gene Full Name extracellular matrix protein 1
Background This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
Function Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. [UniProt]
Cellular Localization Cell surface and cytoplasmic
Calculated MW 61 kDa