ARG42702

anti-EDNRA / Endothelin A receptor antibody

anti-EDNRA / Endothelin A receptor antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes EDNRA / Endothelin A receptor
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EDNRA / Endothelin A receptor
Antigen Species Human
Immunogen Recombinant protein corresponding to N32-Q381 of Human EDNRA / Endothelin A receptor.
Conjugation Un-conjugated
Alternate Names ETA-R; ETAR; Endothelin-1 receptor; ETRA; Endothelin A receptor; MFDA; ET-A; ETA; hET-AR

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HEK293
Observed Size ~ 50 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose.
Preservative 0.01% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1909 Human EDNRA

Swiss-port # P25101 Human Endothelin-1 receptor

Gene Symbol EDNRA
Gene Full Name endothelin receptor type A
Background This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Function Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 49 kDa