ARG43754

anti-EEA1 antibody

anti-EEA1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes EEA1
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EEA1
Antigen Species Human
Immunogen Synthetic peptide corresponding to Human EEA1.
Conjugation Un-conjugated
Alternate Names Early endosome antigen 1; Zinc finger FYVE domain-containing protein 2; MST105; MSTP105; ZFYVE2; Endosome-associated protein p162

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, Placenta, Brain, Spleen, A431
Observed Size ~170 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 216238 Mouse EEA1

GeneID: 8411 Human EEA1

Swiss-port # Q15075 Human Early endosome antigen 1

Swiss-port # Q8BL66 Mouse Early endosome antigen 1

Gene Symbol EEA1
Gene Full Name early endosome antigen 1
Background Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. [UniProt]
Cellular Localization Cytoplasm. Early endosome membrane; Peripheral membrane protein. [UniProt]
Calculated MW 162 kDa