ARG63661

anti-EHD2 antibody

anti-EHD2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes EHD2
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise EHD1 protein as well as EHD2.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name EHD2
Antigen Species Human
Immunogen CRLVPPSKRRHKGSA
Conjugation Un-conjugated
Alternate Names PAST2; EH domain-containing protein 2; PAST homolog 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-PAssay - dependent
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 30846 Human EHD2

Swiss-port # Q9NZN4 Human EH domain-containing protein 2

Background This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 61 kDa