ARG55880

anti-EHMT2 / G9A antibody

anti-EHMT2 / G9A antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes EHMT2
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name EHMT2 / G9A
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 361-395 (Center) of Human EHMT2.
Conjugation Un-conjugated
Alternate Names H3-K9-HMTase 3; GAT8; KMT1C; G9A; Euchromatic histone-lysine N-methyltransferase 2; Histone H3-K9 methyltransferase 3; Protein G9a; NG36; Lysine N-methyltransferase 1C; C6orf30; HLA-B-associated transcript 8; EC 2.1.1.43; Histone-lysine N-methyltransferase EHMT2; EC 2.1.1.-; BAT8

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10919 Human EHMT2

GeneID: 110147 Mouse EHMT2

Swiss-port # Q96KQ7 Human Histone-lysine N-methyltransferase EHMT2

Swiss-port # Q9Z148 Mouse Histone-lysine N-methyltransferase EHMT2

Gene Symbol EHMT2
Gene Full Name euchromatic histone-lysine N-methyltransferase 2
Background This gene is located in a gene cluster within the human major histocompatibility complex class III region on chromosome 6. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but recent publications show that there is only a single gene at this locus. The encoded protein is a histone methyltransferase, which may be involved in repression of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. [UniProt]
Cellular Localization Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin
Calculated MW 132 kDa
PTM Methylated at Lys-185; automethylated.